病毒复制机制与药物开发

1、首次解析了猴痘病毒DNA聚合酶全酶复合物的结构，并揭示了痘病毒聚合酶发挥可持续合成能力的特殊机制，不仅进一步促进了对痘病毒复制过程的理解，同时为开发抗病毒药物应对猴痘疫情提供了分子基础（Science 2023; PNAS 2025）

2、解析了埃博拉病毒聚合酶复合物的结构，阐明了其工作机制，并发现苏拉明能通过抑制底物进入酶活中心而抑制埃博拉病毒聚合酶活性的分子机制 (Nature 2022)；同时还进一步解析了埃博拉病毒聚合酶与基因组启动子复合物的结构，阐明了其发挥从头起始复制活性的分子机制（Nature 2023）

3、主持青年基金，面上项目，获得国家级青年人才项目（2024）。

1、 Yufeng Xie#, Lu Kuai#, Qi Peng#, Qian Wang#, Han Wang, Xiaomei Li, Jianxun Qi, Qiang Ding, Yi Shi*, George F. Gao*. Structural basis of DNA replication fidelity of the Mpox virus. Proceedings of the National Academy of Sciences of the United States of America (2025).

2、 Qi Peng#, Yingying Dong#, Mingzhu Jia#, Qiannv Liu#, Yuhai Bi, Jianxun Qi, Yi Shi*. Cryo-EM structure of Nipah virus L-P polymerase complex. Nature Communications (2024).

3、Qi Peng#, Bin Yuan#, Jinlong Cheng#, Min Wang, Siwei Gao, Suran Bai, Xuejin Zhao, Jianxun Qi*, George F. Gao*, Yi Shi*. Molecular mechanism of de novo replication by the Ebola virus polymerase. Nature , 622, 603-610 (2023).

4、Qi Peng#, Yufeng Xie#, Lu Kuai#, Han Wang, Jianxun Qi, George F. Gao*, Yi Shi*. Structure of monkeypox virus DNA polymerase holoenzyme. Science, 379, 100-105 (2023).

5、Bin Yuan#, Qi Peng#, Jinlong Cheng, Min Wang, Jin Zhong, Jianxun Qi, George F. Gao*, Yi Shi*. Structure of the Ebola virus polymerase complex. Nature, 610, 394-401 (2022).

6、Xin Xu#, Ruchao Peng#, Qi Peng#, Min Wang, Ying Xu, Sheng Liu, Xiaolin Tian, Haiteng Deng, Yimin Tong, Xiaoyou Hu, Jin Zhong, Peiyi Wang, Jianxun Qi, George F. Gao, Yi Shi*. Cryo-EM structures of Lassa and Machupo virus polymerases complexed with cognate regulatory Z proteins identify targets for antivirals. Nature Microbiology, 6, 921-931(2021).

7、Qi Peng#, Ruchao Peng#, Bin Yuan#, Min Wang, Jingru Zhao, Lifeng Fu, Jianxun Qi, Yi Shi*. Structural Basis of SARS-CoV-2 Polymerase Inhibition by Favipiravir. The Innovation, 2(1), 100080 (2021).

8、Qi Peng#, Ruchao Peng#, Bin Yuan#, Jingru Zhao#, Min Wang, Xixi Wang, Qian Wang, Yan Sun, Zheng Fan, Jianxun Qi, George Fu Gao and Yi Shi*. Structural and biochemical characterization of nsp12-nsp7-nsp8 core polymerase complex from SARS-CoV-2. Cell Reports. 31, 107774 (2020).

9、Huahao Fan#, Bixia Hong#, Yuqian Luo#, Qi Peng#, Liqin Wang, Xiangshu Jin, Yangzhen Chen, Yunjia Hu, Yi Shi, Tong Li, Hui Zhuang, Yi-Hua Zhou*, Yigang Tong*, Kuanhui Xiang*. The effect of whey protein on viral infection and replication of SARS-CoV-2 and pangolin coronavirus in vitro. Signal Transduction and Targeted Therapy, 5(1), 275, (2020).

10、Qi Peng#, Yuqian Liu, Ruchao Peng, Min Wang, Wei Yang, Hao Song, Yuhai Chen, Sheng Liu, Min Han, Xinzheng Zhang, Peiyi Wang, Jinghua Yan, Buchang Zhang, Jianxun Qi, Tao Deng, George F. Gao*, Yi Shi*. Structural insight into RNA synthesis by influenza D polymerase. Nature Microbiology, 4, 1950-1957 (2019).

11、Zhenyang Liu#, Qi Peng#, Pu Han#, Lu Kuai, Jianxun Qi, Yi Shi*. Crystal structures of RNA-dependent RNA polymerases from Jingmen tick virus and Alongshan virus. hLife , 2, 18-31(2024).